Can a used ECM be programmed with ODIS?

yes

No problem at all!

ODIS does allow the use of used ECM's, but many shops run the wrong ODIS test plan when doing so. Always ensure you use the Immobilizer Serving test plan which is typically located in ODIS under the Special Functions tab, then run the ECM replacement test plan which is located under the ECM, Guided Fault Finding. Please ensure you answer the questions correctly during the test plan. Many users…

All day long

My experience is that the replacement has to be the same part number using the same transmission. Especially if a SIMOS ECU. If that is all correct, it is a fairly simple process with ODIS as mentioned by others.